Cell-substrate interactions in amoeboid locomotion - a matched reflexion interference and transmission electron microscopy study.

Cell-substrate separation distance were measured on Naegleria gruberi amoebae moving in deionized H2O on an untreated glass substratum (weakly adhesive) and on a polylysine treated glass surface (strongly adhesive). The values obtained by transmission electron microscopy on fixed cells and reflexion interference microscopy on live cells were in broad agreement.