氧气供应减少可促进孕中期人羊水细胞培养中的生长。
Decreased oxygen supply enhances growth in culture of human mid-trimester amniotic fluid cells.
作者信息
Brackertz M, Kubbies M, Feige A, Salk D
出版信息
Hum Genet. 1983;64(4):334-8. doi: 10.1007/BF00292364.
PMID:6618486
Abstract
Human mid-trimester amniotic fluid cells were cultivated under conditions of decreased oxygen supply. Compared to control cultures the low-oxygen group showed improved growth which was quantitated by three independent assays (1) direct cell counts, (2) bromodeoxyuridine (BrdU)-Hoechst flow-cytometry, and (3) cloning efficiency. The growth promoting effects of lowered oxygen hold for all major morphologic categories of amniotic fluid cells.
摘要
人孕中期羊水细胞在低氧供应条件下进行培养。与对照培养相比，低氧组的生长情况有所改善，通过三种独立检测方法进行定量分析：(1) 直接细胞计数；(2) 溴脱氧尿苷（BrdU）-Hoechst 流式细胞术；(3) 克隆效率。降低氧含量对羊水细胞的所有主要形态学类别均有促进生长的作用。
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