Stroo W E, Hook J B
J Pharmacol Exp Ther. 1983 Oct;227(1):55-9.
The objective of this study was to evaluate the hypothesis that nonesterified fatty acids (NEFA) are endogenous inhibitors of renal organic anion transport and that changes in renal NEFA content could modulate renal organic anion transport capacity. The addition of 1 mM palmitate to a suspension of renal tubules produced a tissue NEFA content of 665 +/- 105 micrograms/g and a 40% decrease in the tissue-to-medium concentration ratio for p-aminohippurate. Penicillin pretreatment enhanced p-aminohippurate tissue-to-medium concentration ratio in a neonatal rabbit proximal tubule suspension but failed to alter renal NEFA content from a control of 46.4 +/- 2.6 micrograms/g. Penicillin treatment did, however, decrease renal triglyceride content and increased serum NEFA from 87.7 +/- 3.4 to 129 +/- 6.8 micrograms/ml. Fasting increased serum triglyceride and increased serum NEFA from 33.9 +/- 3.1 to 370 +/- 200 micrograms/ml. Fasting decreased p-aminohippurate transport capacity 42% from a control tissue-to-medium concentration ratio of 6.78 +/- 1.2 but did not alter renal NEFA from a control value of 65 +/- 15 micrograms/g. The data support the suggestion that penicillin treatment can alter lipid metabolism in vivo but fail to support the suggestion that altered renal organic anion transport is due to altered renal NEFA content and further suggest that NEFA can alter renal organic anion transport only at a supraphysiological concentration.
非酯化脂肪酸（NEFA）是肾脏有机阴离子转运的内源性抑制剂，并且肾脏NEFA含量的变化可调节肾脏有机阴离子转运能力。向肾小管悬浮液中添加1 mM棕榈酸酯可使组织NEFA含量达到665±105微克/克，并使对氨基马尿酸的组织与介质浓度比降低40%。青霉素预处理可提高新生兔近端肾小管悬浮液中对氨基马尿酸的组织与介质浓度比，但未能改变肾脏NEFA含量，对照组为46.4±2.6微克/克。然而，青霉素治疗确实降低了肾脏甘油三酯含量，并使血清NEFA从87.7±3.4微克/毫升增加至129±6.8微克/毫升。禁食可增加血清甘油三酯，并使血清NEFA从33.9±3.1微克/毫升增加至370±200微克/毫升。禁食使对氨基马尿酸的转运能力从对照组的组织与介质浓度比6.78±1.2降低了42%，但未改变肾脏NEFA含量，对照组值为65±15微克/克。数据支持青霉素治疗可在体内改变脂质代谢的观点，但不支持肾脏有机阴离子转运改变是由于肾脏NEFA含量改变的观点，并且进一步表明NEFA仅在超生理浓度下才能改变肾脏有机阴离子转运。
