Webber M M, Horan P K, Bouldin T R
Invest Urol. 1977 Mar;14(5):335-43.
This report reviews the background and the present status of the MA-160 cell line. This cell line was reported to have arisen from tissue cultures of a human, benign prostatic adenoma and consequently has been used for investigations on the prostat and on hormone-dependent cells. Recent studies using chromosome analysis by banding techniques, glucose-6-phosphate dehydrogenase (G6D) electrophoretic mobility, and a specific prostatic acid phosphatase test indicate that MA-160 is not of prostatic origin but a HeLa cell contaminant, arising as a result of intraspecies cell contamination. It is suggested that whenever spontaneous transformation is observed in vitro and established cell lines are being used in the laboratory, the cells in question be thoroughly checked with the currently available chromosomal, biochemical, and cytochemical methods in order to establish their origin and to rule out interspecies and intraspecies cell contamination.
本报告回顾了MA - 160细胞系的背景和现状。据报道，该细胞系源自一名人类良性前列腺腺瘤的组织培养物，因此一直被用于前列腺及激素依赖性细胞的研究。最近利用染色体显带技术分析、葡萄糖 - 6 - 磷酸脱氢酶（G6D）电泳迁移率以及特异性前列腺酸性磷酸酶检测的研究表明，MA - 160并非源自前列腺，而是一种HeLa细胞污染物，是种内细胞污染的结果。建议在体外观察到自发转化且实验室正在使用已建立的细胞系时，用现有的染色体、生化和细胞化学方法对相关细胞进行彻底检查，以确定其来源并排除种间和种内细胞污染。
