利用单克隆抗体对与人类11号染色体相关的杂交细胞表面抗原进行的进一步研究。

Further studies on a hybrid cell-surface antigen associated with human chromosome 11 using a monoclonal antibody.

作者信息

Jones C, Kimmel K A, Carey T E, Miller Y E, Lehman D W, MacKenzie D

出版信息

Somatic Cell Genet. 1983 Jul;9(4):489-96. doi: 10.1007/BF01543049.

Abstract

A monoclonal antibody has been obtained that recognizes an antigen encoded by human chromosome 11. We present evidence that this monoclonal antibody recognizes the same or a similar antigenic activity as that previously called a1. Genetic information necessary for a1 expression and recognition by the monoclonal antibody both map to 11p13 leads to 11pter. Mutants that have lost a1 are no longer recognized by the monoclonal antibody. The macroglycolipid fraction of human erythrocyte membranes which contains the a1 antigenic activity is able to convert antigen-negative Chinese hamster ovary cells into cells which are killed by the monoclonal antibody plus complement.

摘要

已获得一种单克隆抗体，它能识别由人类11号染色体编码的一种抗原。我们提供的证据表明，这种单克隆抗体识别的抗原活性与先前称为a1的抗原活性相同或相似。a1表达以及被该单克隆抗体识别所必需的遗传信息都定位于11p13至11pter区域。失去a1的突变体不再被该单克隆抗体识别。含有a1抗原活性的人红细胞膜的大糖脂部分能够将抗原阴性的中国仓鼠卵巢细胞转化为可被该单克隆抗体加补体杀死的细胞。