Komulainen H
Acta Pharmacol Toxicol (Copenh). 1983 Aug;53(2):166-74. doi: 10.1111/j.1600-0773.1983.tb01886.x.
Lead (0.2%), zinc (0.5%) or their combination was given to rabbits as acetate salts in drinking water for 2 or 4 weeks. Cerebral Pb increased within exposure time but the increase of Zn was marginal when given alone. After combination of Pb + Zn, blood Pb increased but Zn decreased as compared to the administration of the single metal alone. Concurrently brain Pb increased but remained at a lower level as compared with sole Pb administration while brain Zn did not differ from controls. The uptake of 5-hydroxytryptamine (5-HT) into hypothalamic synaptosomes increased 70-85% with Pb, Zn and Pb + Zn after 2 weeks and 93% with Zn and 76% with Pb + Zn after 4 weeks. Dopamine uptake into striatal synaptosomes did not change. Zn decreased endogenous noradrenaline concentrations by 21% and Pb + Zn that of 5-HT by 36% in the striatum. Pb + Zn decreased cortical dopamine concentrations by 26%. Concurrently, 5-HT uptake in blood platelets, platelet numbers and their content of endogenous 5-HT did not change significantly. These results indicate that high Zn and Pb have parallel effects on 5-HT uptake in hypothalamic synaptosomes and their combination may be neurochemically more toxic than the individual metals. The increase of 5-HT uptake in hypothalamic synaptosomes but not in blood platelets requires further methodological work to explain the differences between synaptosomes and platelets.
将醋酸盐形式的铅（0.2%）、锌（0.5%）或它们的组合添加到家兔饮用水中，持续2周或4周。在暴露期间，大脑中的铅含量增加，但单独给予锌时，锌的增加幅度很小。铅与锌联合使用后，与单独给予单一金属相比，血液中的铅含量增加而锌含量降低。同时，大脑中的铅含量增加，但与单独给予铅相比仍处于较低水平，而大脑中的锌含量与对照组无差异。2周后，下丘脑突触体对5-羟色胺（5-HT）的摄取在铅、锌和铅 + 锌作用下增加70 - 85%，4周后，锌作用下增加93%，铅 + 锌作用下增加76%。纹状体突触体对多巴胺的摄取没有变化。锌使纹状体内内源性去甲肾上腺素浓度降低21%，铅 + 锌使5-HT浓度降低36%。铅 + 锌使皮质多巴胺浓度降低26%。同时，血小板对5-HT的摄取、血小板数量及其内源性5-HT含量没有明显变化。这些结果表明，高浓度的锌和铅对下丘脑突触体中5-HT的摄取有相似作用，它们的组合在神经化学方面可能比单一金属更具毒性。下丘脑突触体中5-HT摄取增加而血小板中未增加，这需要进一步的方法学研究来解释突触体和血小板之间的差异。
