Sahebjami H, MacGee J
Am Rev Respir Dis. 1983 Oct;128(4):644-7. doi: 10.1164/arrd.1983.128.4.644.
Adult male rats were starved by allowing them one fifth of their measured daily food consumption until they lost 40% of their initial body weights. Some of these rats were then refed until their initial body weights were reached. We measured the total content of the following in the lung tissue of fed, starved, and refed animals: (1) elastin, (2) hydroxyproline, and (3) protein. Body weight and lung dry and wet weights were significantly reduced in starved and similar in refed rats compared with fed animals. Total contents of crude connective tissue, hydroxyproline, elastin, and protein were significantly lower in starved than in fed rat lungs. After refeeding, hydroxyproline content returned completely to levels found in fed rats, but other components only partially returned to normal values. These results provide a biochemical counterpart for our previous observations on the effects of starvation and refeeding on lung mechanics and morphologic aspects. It appears that the emphysema like changes in the lungs of starved rats are at least partly related to the loss of connective tissue elements.
成年雄性大鼠被限制进食，仅给予其测量出的每日食物消耗量的五分之一，直至体重减轻40%。然后，其中一些大鼠重新喂食，直至恢复到初始体重。我们测量了喂食、饥饿和重新喂食的动物肺组织中以下物质的总含量：（1）弹性蛋白，（2）羟脯氨酸，（3）蛋白质。与喂食的动物相比，饥饿大鼠的体重以及肺的干湿重量显著降低，重新喂食的大鼠则与之相似。饥饿大鼠肺中粗结缔组织、羟脯氨酸、弹性蛋白和蛋白质的总含量显著低于喂食大鼠。重新喂食后，羟脯氨酸含量完全恢复到喂食大鼠的水平，但其他成分仅部分恢复到正常值。这些结果为我们之前关于饥饿和重新喂食对肺力学和形态学影响的观察提供了生化方面的对应依据。饥饿大鼠肺部类似肺气肿的变化似乎至少部分与结缔组织成分的丧失有关。
