用大鼠粪便的无细胞提取物去除大鼠结肠上皮糖蛋白上的O-乙酰化唾液酸。

Removal of O-acetylated sialic acids from rat colonic epithelial glycoproteins by cell-free extracts of rat faeces.

作者信息

Poon H, Reid P E, Ramey C W, Dunn W L, Clay M G

出版信息

Can J Biochem Cell Biol. 1983 Aug;61(8):868-74. doi: 10.1139/o83-111.

Abstract

Sterile, cell-free, extracts of freshly defaecated Wistar rat faeces in a pH 7.0 "minimal medium" contain neuraminidase(s), capable of removing sialic acids both with and without side-chain substituents from bovine submandibular mucin and rat colonic epithelial glycoproteins, and an esterase which removes O-acetyl substituents from the side chain of sialic acid residues. Studies of the removal of sialic acids from bovine submandibular mucin and rat colonic epithelial glycoproteins indicated that (i) the faecal enzymes removed a greater proportion of the sialic acid of both the de-O-acetylated and native glycoproteins than was removed with Vibrio cholera neuraminidase, (ii) sialic acids were removed more rapidly from de-O-acetylated glycoproteins, and (iii) the resistance to removal of sialic acids was apparently dependent at least in part upon the O-acetyl sialic acid content of the substrate.

摘要

在pH 7.0的“基本培养基”中，新鲜排出的Wistar大鼠粪便的无菌无细胞提取物含有神经氨酸酶，该酶能够从牛下颌粘蛋白和大鼠结肠上皮糖蛋白中去除带有和不带有侧链取代基的唾液酸，还含有一种酯酶，可从唾液酸残基的侧链上去除O - 乙酰取代基。对从牛下颌粘蛋白和大鼠结肠上皮糖蛋白中去除唾液酸的研究表明：（i）粪便酶从去O - 乙酰化和天然糖蛋白中去除的唾液酸比例比霍乱弧菌神经氨酸酶去除的比例更高；（ii）唾液酸从去O - 乙酰化糖蛋白中去除得更快；（iii）唾液酸去除的抗性显然至少部分取决于底物的O - 乙酰唾液酸含量。