Elkins W L, Klinman N R, Mayol R
J Immunol. 1977 Mar;118(3):998-1003.
An indirect radioimmunoassay was used to detect Ia antigenic determinants at the cell surface of thymic and splenic cells. An A.TH anti-A.TL serum was used as a source of anti-Ia antibodies which could bind to H-2a or H-2k target cells. Bound alloantibody was then detected with 125I anti-mouse IgG1 antibody. This assay system revealed Ia antigens on thymic T cells which are not usually detectable by cytotoxicity assays, and has the potential for quantitative measurement of such determinants on cells. Critical adsorption studies were performed to confirm that Ia antigens were in fact the targets of the alloantiserum tested.
采用间接放射免疫测定法检测胸腺细胞和脾细胞表面的Ia抗原决定簇。使用A.TH抗A.TL血清作为抗Ia抗体的来源，该抗体可与H-2a或H-2k靶细胞结合。然后用125I抗小鼠IgG1抗体检测结合的同种异体抗体。该检测系统揭示了胸腺T细胞上的Ia抗原，而细胞毒性检测通常无法检测到这些抗原，并且有可能对细胞上的此类决定簇进行定量测量。进行了关键吸附研究以证实Ia抗原实际上是所测试的同种异体抗血清的靶标。
