Bevan M J
J Immunol. 1977 Apr;118(4):1370-4.
Spleen cells from normal mice do not give a detectable in vitro cytotoxic T cell (CTL) response to minor H antigens. Spleen cells from animals primed in vivo with minor H antigens give a strong CTL response when boosted in culture with the appropriate stimulating cells. Here I have studied the requirements for priming a CTL response to minor H antigens. It is shown that priming is just as antigen specific as is cytolytic effector function. That is, priming cells have to carry the same minor antigens as the challenge cells. Inducing a graft-vs-host reaction in vivo does not nonspecifically allow spleen cells to respond to minor H antigens in vitro. Using minor H congenic mice (congenic for H-Y and/or H-7) I have also tried, and failed, to demonstrate a carrier effect in priming. Female mice primed to H-Y were challenged in culture with cells bearing H-Y and H-7 antigens in the hope that a helper response to H-Y would augment a CTL response to H-7. This did not happen, however. Such primed and boosted cells gave a strong secondary CTL response to H-Y but none to H-7. It is concluded that in order to prime for a detectable in vitro response to minor antigens it is necessary to expose the CTL precursors to antigen in vivo. This either expands the size of the pool of precursors by cell division or changes them in some qualitative way.
正常小鼠的脾细胞对次要组织相容性抗原不会产生可检测到的体外细胞毒性T细胞（CTL）应答。用次要组织相容性抗原在体内进行免疫的动物的脾细胞，在与适当的刺激细胞一起培养时会产生强烈的CTL应答。在此，我研究了引发针对次要组织相容性抗原的CTL应答的条件。结果表明，引发与溶细胞效应功能一样具有抗原特异性。也就是说，引发细胞必须携带与攻击细胞相同的次要抗原。在体内诱导移植物抗宿主反应并不能非特异性地使脾细胞在体外对次要组织相容性抗原产生应答。利用次要组织相容性同基因小鼠（H-Y和/或H-7同基因），我也尝试证明引发过程中的载体效应，但未成功。用H-Y免疫的雌性小鼠在培养中用携带H-Y和H-7抗原的细胞进行攻击，希望对H-Y的辅助应答能增强对H-7的CTL应答。然而，这并未发生。这种经引发和再次刺激的细胞对H-Y产生强烈的二次CTL应答，但对H-7无应答。结论是，为了引发针对次要抗原的可检测到的体外应答，有必要在体内使CTL前体细胞接触抗原。这要么通过细胞分裂扩大前体细胞池的大小，要么以某种定性方式改变它们。
