Dresler S L, Lieberman M W
J Biol Chem. 1983 Oct 25;258(20):12269-73.
Studies from several laboratories have shown that ATP is required for DNA excision repair in UV-irradiated mammalian cells. Using permeable human fibroblasts, we have investigated this ATP requirement in detail. We find that ATP is required for specific incision of UV-damaged DNA in permeable cells. No ATP-dependent incision is seen in UV-irradiated permeable xeroderma pigmentosum (complementation group G) fibroblasts, indicating that the ATP-dependent incision observed in normal cells is part of the normal excision repair process. We conclude that, in mammalian cells, ATP is required for specific incision of UV-damaged DNA or for some obligatory step preceding incision in the excision repair pathway. ATP also protects the permeable cells from loss of the capacity to perform excision repair, probably in a nonspecific fashion. The actual synthesis of repair patches can proceed in the absence of ATP; however, our data do not exclude the possibility that ATP can also stimulate repair synthesis directly.
来自多个实验室的研究表明，在紫外线照射的哺乳动物细胞中，DNA切除修复需要ATP。我们使用可渗透的人成纤维细胞，详细研究了这种对ATP的需求。我们发现，在可渗透细胞中，ATP是紫外线损伤DNA特异性切割所必需的。在紫外线照射的可渗透着色性干皮病（互补组G）成纤维细胞中未观察到ATP依赖性切割，这表明在正常细胞中观察到的ATP依赖性切割是正常切除修复过程的一部分。我们得出结论，在哺乳动物细胞中，ATP是紫外线损伤DNA特异性切割所必需的，或者是切除修复途径中切割前的某些必要步骤所必需的。ATP还可能以非特异性方式保护可渗透细胞免于丧失进行切除修复的能力。修复补丁的实际合成可以在没有ATP的情况下进行；然而，我们的数据并不排除ATP也可以直接刺激修复合成的可能性。
