Castejón O J
Scan Electron Microsc. 1983(Pt 1):151-60.
Samples of teleost cerebellar cortex (Arius Spixii and Salmo Trout) fixed by immersion and vascular perfusion techniques were processed for light microscopy, SEM and TEM. SEM fractographs of endothelial cell nuclear, organelle and peripheral cytoplasmic zones have been compared with their corresponding TEM images. A simultaneous three-dimensional view of the luminal surface of endothelial cells at the nuclear zone, and inner details of heterochromatin structure was obtained. Chained micropinocytotic vesicles and deep cytoplasmic invaginations were observed. Surface connected micropinocytotic vesicles or vacuoles show stomas open to the luminal surface. Clear and dense endothelial micropinocytotic vesicles and vacuoles were observed at TEM level. The dense plasma substance can be used as an endogenous electron dense tracer for permeability studies. At SEM level the rough endoplasmic reticulum appears as a trabecular continuous system. The endothelial cytosol exhibits a smooth, glass surface appearance. The endothelial luminal surface changes its aspect according to the fixation procedure. The SEM and TEM aspect of endothelial junctions further supports their role as the morphological counterpart of blood-brain barrier. The basement membrane exhibited a homogeneous matrix and short or long neuropilar projections. Clear perivascular astrocytes with anastomotic processes form the main framework of perivascular neuropile. Like the peripheral endothelial cytoplasm, they exhibited dense and clear micropinocytotic vesicles and vacuoles, providing evidence for their transport function between capillaries and neighbouring brain parenchyma. The SEM appearance of their outer surface further supports their barrier function.
采用浸入法和血管灌注技术固定的硬骨鱼小脑皮质样本（阿里乌斯鲇和鲑鱼），用于光学显微镜、扫描电子显微镜（SEM）和透射电子显微镜（TEM）检查。已将内皮细胞核、细胞器和外周细胞质区域的SEM断口图与其相应的TEM图像进行了比较。获得了核区内皮细胞腔表面的同时三维视图以及异染色质结构的内部细节。观察到链状微胞饮小泡和深细胞质内陷。表面相连的微胞饮小泡或液泡显示有通向腔表面的小孔。在TEM水平观察到清晰和致密的内皮微胞饮小泡和液泡。致密的血浆物质可作为内源性电子致密示踪剂用于通透性研究。在SEM水平，粗面内质网呈现为小梁状连续系统。内皮细胞质溶胶呈现出光滑的玻璃表面外观。内皮腔表面根据固定程序改变其外观。内皮连接的SEM和TEM外观进一步支持了它们作为血脑屏障形态学对应物的作用。基底膜呈现出均匀的基质和短或长的神经纤维突起。具有吻合过程的清晰的血管周围星形胶质细胞形成血管周围神经纤维的主要框架。与外周内皮细胞质一样，它们也表现出致密和清晰的微胞饮小泡和液泡，为它们在毛细血管和邻近脑实质之间的转运功能提供了证据。它们外表面的SEM外观进一步支持了它们的屏障功能。
