Harada Y, Hirakawa K, Mangape D
Scan Electron Microsc. 1983(Pt 1):183-8.
Scanning electron microscopy (SEM) study of the innervation of the organ of Corti has been carried out for many years. Various methods have been used in attempts to observe more details of the endings of nerve fibers on the sensory hair cells. In this study, a new method of microdissection of Corti's organ was done using a small piece of double-sided adhesive tape. This method is simple and facilitates observation of Corti's organ from the basal to the apical turns. Using it we found variations of the nerve endings and the Deiters' cells in each half turn beginning from the lower basal turn to the upper fourth turn. Nerve endings at the lower poles of outer hair cells are arranged in clusters, and are reduced in size and number as the upper turn is approached. On the contrary, the Deiters' cells are increased in size toward the upper turn, therefore nerve endings in the upper turn are often covered by Deiters' cells. Small nerve endings high on the side of the outer hair cell were also observed. Although there was clear evidence of ultrastructural change in the hair cells' cilia after administration of ototoxic drugs or after hyperstimulation by sound, there was no evidence of concomitant ultrastructural change in the nerve endings. Nerve endings appeared to be more resistant than the hair cells.
多年来一直在进行对柯蒂氏器神经支配的扫描电子显微镜（SEM）研究。人们采用了各种方法试图观察神经纤维在感觉毛细胞上的末梢的更多细节。在本研究中，使用一小片双面胶带对柯蒂氏器进行了一种新的显微解剖方法。该方法简单，便于从基部到顶部观察柯蒂氏器。使用这种方法，我们发现从基部较低的半圈到顶部第四半圈，每半圈神经末梢和Dieters细胞都有变化。外毛细胞下极的神经末梢成簇排列，随着接近上半圈，其大小和数量会减少。相反，Dieters细胞向上半圈方向尺寸增大，因此上半圈的神经末梢常被Dieters细胞覆盖。还观察到外毛细胞侧面较高位置有小的神经末梢。尽管在给予耳毒性药物后或在声音过度刺激后，毛细胞纤毛有明显的超微结构变化证据，但神经末梢没有伴随超微结构变化的证据。神经末梢似乎比毛细胞更具抗性。
