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[丙酸盐在大鼠胆碱合成中作用的直接证据]

[Direct evidence of the role of propionate in choline synthesis in rats].

作者信息

Astvatsatur'ian A T, Staviskiĭ I M, Alimova E K

出版信息

Biull Eksp Biol Med. 1983 Nov;96(11):38-9.

PMID:6640095
Abstract

Wistar rats were injected with 2-14C-propionate in a dose of 30 mu Ci/100 g bw, 2 h after food intake. Two hours after isotope injection the rats were decapitated to determine specific radioactivity (SR) in liver and brain lipids, in liver phosphatidylcholine (PC) and its structural components. The label was incorporated in liver lipids in a far greater amount. In liver PC, SR appeared the highest in glycerin and less higher in the fraction of higher fatty acids. The least amount of the label from 2-14C-propionate was incorporated in choline. The fact of the label incorporation in choline was recorded for the first time.

摘要

在进食2小时后,给Wistar大鼠注射剂量为30微居里/100克体重的2-¹⁴C-丙酸盐。同位素注射两小时后,将大鼠断头以测定肝脏和脑脂质、肝脏磷脂酰胆碱(PC)及其结构成分中的比放射性(SR)。标记物在肝脏脂质中的掺入量要多得多。在肝脏PC中,甘油中的SR最高,在高级脂肪酸部分中的SR略低。2-¹⁴C-丙酸盐中标记物掺入胆碱的量最少。首次记录到标记物掺入胆碱这一事实。

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