[人骨髓基质成纤维细胞克隆的细胞化学特征]
[Cytochemical characteristics of the clones of human bone marrow stromal fibroblasts].
作者信息
Astakhova V S, Gluzman D F, Poludnenko L Iu
出版信息
Biull Eksp Biol Med. 1983 Nov;96(11):95-7.
PMID:6640104
Abstract
Cytochemical methods were used to study the activity of acid and alkaline phosphatases, neutral and acid nonspecific esterases, and peroxidase in the clones of stromal human marrow stromal fibroblasts at the 10th-12th day of cultivation with rabbit feeder. All the cells in the clones contained acid phosphatase. All the colonies had 5 to 90% of cells with positive response to alkaline phosphatase. The colony cells did not show any neutral and acid nonspecific esterases or peroxidase.
摘要
采用细胞化学方法研究了人骨髓基质成纤维细胞克隆在与兔饲养层共培养第10 - 12天时酸性和碱性磷酸酶、中性和酸性非特异性酯酶以及过氧化物酶的活性。克隆中的所有细胞均含有酸性磷酸酶。所有集落中有5%至90%的细胞对碱性磷酸酶呈阳性反应。集落细胞未显示任何中性和酸性非特异性酯酶或过氧化物酶。
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