Barakat I, Massarelli R, Courageot J, Devilliers G, Sensenbrenner M
Brain Res. 1983 Nov 21;279(1-2):207-16. doi: 10.1016/0006-8993(83)90179-8.
Cells dissociated from cerebral hemispheres of 6-day-old chick embryos were cultured either in standard nutrient medium or in the presence of a brain extract from 8-day-old chick embryo. Morphological observations showed the development of bipolar and multipolar neurons in both culture conditions and acetylcholinesterase activity was found in all neuronal cells. Brain extract stimulated the morphological maturation of neurons, expressed by the formation of fiber bundles, fine structural maturation and development of synapses rich in clear vesicles. Furthermore, acetylcholinesterase and choline acetyltransferase activities were higher in the cultures treated with brain extract. In these cultures, the values of choline acetyltransferase activity reached a peak at 10 days and then decreased. These observations are discussed with particular reference to proliferation, maturation and degeneration of cholinergic neurons.
从6日龄鸡胚大脑半球分离出的细胞，分别在标准营养培养基中或在8日龄鸡胚脑提取物存在的情况下进行培养。形态学观察显示，在两种培养条件下均有双极和多极神经元发育，并且在所有神经元细胞中均发现了乙酰胆碱酯酶活性。脑提取物刺激了神经元的形态成熟，表现为纤维束的形成、精细结构的成熟以及富含清亮小泡的突触的发育。此外，用脑提取物处理的培养物中乙酰胆碱酯酶和胆碱乙酰转移酶的活性更高。在这些培养物中，胆碱乙酰转移酶活性值在第10天达到峰值，然后下降。结合胆碱能神经元的增殖、成熟和退化对这些观察结果进行了讨论。
