Megalinskaia A P, Skobets E M, Lishko V K, Grinevich V V
Ukr Biokhim Zh (1978). 1978 May-Jun;50(3):377-80.
A method is suggested for a quantitative determination of sulphydryl and imidazole groups in proteins. The method is based on the capacity of Zn2+ and Co2+ to bind selectivity with sulphur and nitrogen atoms in bioligands. The mentioned reactions are fixed by one of most sensitive methods of the electrochemical analysis, the inversiochronopotentiometric titration. The method was tested with the solutions of amino acids and proteins. The sensitivity of the method is 10(-8)M, a relative error is +/- 1.5%.
提出了一种定量测定蛋白质中巯基和咪唑基的方法。该方法基于Zn2+和Co2+与生物配体中的硫和氮原子选择性结合的能力。上述反应通过电化学分析中最灵敏的方法之一——反向计时电位滴定法来确定。该方法已用氨基酸和蛋白质溶液进行了测试。该方法的灵敏度为10(-8)M，相对误差为±1.5%。
