Fairweather I, Holmes S D, Threadgold L T
Exp Parasitol. 1983 Dec;56(3):369-80. doi: 10.1016/0014-4894(83)90082-6.
An apparatus utilizing a force and displacement transducer is described for the direct and long-term recording of the motility in vitro of Fasciola hepatica. Normal movement is typically rhythmical, with bursts of more powerful contractions alternating with periods of lesser activity. Such rhythms and the overall level of activity are maintained for more than 30 hr. The fluke remains active for much longer periods of time: recordings of fluke movements have been made for up to 4 days. Potential damage to the fluke caused by the attachment system within the recording apparatus has been determined by the Evans' Blue Technique and scanning electron microscopy. It is restricted to the attachment sites, and does not spread to other parts of the body over the 30-hr normal activity period. Transmission electron microscope studies have shown that the tegument retains its structural and functional integrity over this period of time. There are advantages of the recording apparatus over previous kymographic methods for studying fluke motility.
描述了一种利用力和位移传感器的装置，用于直接和长期记录肝片吸虫的体外运动。正常运动通常是有节律的，较强有力的收缩爆发与活动较少的时期交替出现。这样的节律和总体活动水平可维持30多个小时。吸虫在更长的时间段内保持活跃：已对吸虫运动进行了长达4天的记录。记录装置内的附着系统对吸虫造成的潜在损伤已通过伊文思蓝技术和扫描电子显微镜确定。它仅限于附着部位，在30小时的正常活动期内不会扩散到身体的其他部位。透射电子显微镜研究表明，在此时间段内，体表保持其结构和功能的完整性。与以前用于研究吸虫运动的记纹鼓方法相比，该记录装置具有优势。
