Waldner-Sander S, Keller B, Keller E, Lingens F
Hoppe Seylers Z Physiol Chem. 1983 Oct;364(10):1467-73.
The enzymes of the terminal steps of phenylalanine and tyrosine biosynthesis, chorismate mutase, prephenate dehydratase, arogenate dehydratase, prephenate dehydrogenase and arogenate dehydrogenase, were studied in 11 different species of the genus Flavobacteria. A comparison of the specific activities, cofactor specificity and regulation of the enzymes, allows a differentiation within the Flavobacteria. All strains studied utilize both arogenate and p-hydroxyphenylpyruvate as an intermediate in L-tyrosine synthesis. Phenylpyruvate was found to be the precursor of phenylalanine in most bacteria. No feedback inhibition of arogenate dehydrogenase by phenylalanine and tyrosine was observed. The diverse strains of the flavobacteria were found to possess different regulatory patterns with respect to the action of phenylalanine and tyrosine on the other enzymes. On the basis of these results a tentative classification of the Flavobacteria within the two groups formed by the different DNA base ratios is proposed.
在11种不同的黄杆菌属物种中，研究了苯丙氨酸和酪氨酸生物合成终末步骤的酶，即分支酸变位酶、预苯酸脱水酶、预苯酸脱氢酶和预苯酸脱氢酶。对这些酶的比活性、辅因子特异性和调节进行比较，可以区分黄杆菌属内的不同种类。所有研究的菌株都利用预苯酸和对羟基苯丙酮酸作为L-酪氨酸合成的中间体。在大多数细菌中，苯丙酮酸被发现是苯丙氨酸的前体。未观察到苯丙氨酸和酪氨酸对预苯酸脱氢酶的反馈抑制作用。发现黄杆菌属的不同菌株在苯丙氨酸和酪氨酸对其他酶的作用方面具有不同的调节模式。基于这些结果，提出了在由不同DNA碱基比率形成的两组内对黄杆菌属进行初步分类的建议。
