Frank R N, Keirn R J, Kennedy A, Frank K W
Invest Ophthalmol Vis Sci. 1983 Nov;24(11):1519-24.
Normotensive and spontaneously hypertensive rats fed a 30% galactose diet until 15-21 months of age developed significant (P less than 0.05) retinal capillary basement membrane thickening, compared with animals fed a standard test diet. Animals on the high galactose diet containing 250 mg/kg of the aldose reductase inhibitor, Sorbinil, did not develop basement membrane thickening. No cytologic abnormalities of pericytes or endothelial cells were noted, and pericyte:endothelial cell nuclear ratios did not differ in the various experimental groups. This model reproduces a characteristic lesion of diabetes mellitus in non-diabetic animals, and should facilitate study of the biochemical mechanisms of basement membrane thickening.
正常血压和自发性高血压大鼠在15至21个月大时喂食30%半乳糖饮食，与喂食标准试验饮食的动物相比，出现了显著（P小于0.05）的视网膜毛细血管基底膜增厚。喂食含250mg/kg醛糖还原酶抑制剂索比尼尔的高半乳糖饮食的动物未出现基底膜增厚。未观察到周细胞或内皮细胞的细胞学异常，不同实验组的周细胞：内皮细胞核比率也无差异。该模型在非糖尿病动物中再现了糖尿病的特征性病变，应有助于研究基底膜增厚的生化机制。
