骨提取物对软骨特异性大分子的体外诱导作用。
In vitro induction of cartilage-specific macromolecules by a bone extract.
作者信息
Seyedin S M, Thompson A Y, Rosen D M, Piez K A
出版信息
J Cell Biol. 1983 Dec;97(6):1950-3. doi: 10.1083/jcb.97.6.1950.
Abstract
An in vitro system has been developed to study the onset of chondrogenesis. Embryonic rat muscle mesenchymal cells, when treated in suspension culture with an extract of bovine bone matrix, synthesized cartilage-specific proteoglycan and type II collagen. The synthesis of these two macromolecules was assayed by the enzyme-linked immunosorbent assay inhibition technique. Further evidence of chondrogenesis was demonstrated by morphological changes of treated cells when cultured in firm agarose and stained for metachromatic matrix. Even with crude bone matrix extracts, the assay was sensitive at the microgram level and significant differences in cartilage macromolecules compared with controls were observed in 2-3 d. In vivo the same extract induced first cartilage and then bone.
摘要
已开发出一种体外系统来研究软骨形成的起始。当胚胎大鼠肌肉间充质细胞在悬浮培养中用牛骨基质提取物处理时，会合成软骨特异性蛋白聚糖和II型胶原蛋白。通过酶联免疫吸附测定抑制技术检测这两种大分子的合成。当在坚硬的琼脂糖中培养并用异染基质染色时，处理过的细胞的形态变化证明了软骨形成的进一步证据。即使使用粗制的骨基质提取物，该测定在微克水平上也很灵敏，并且在2 - 3天内观察到与对照相比软骨大分子有显著差异。在体内，相同的提取物首先诱导形成软骨，然后形成骨。
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