Syftestad G T, Triffitt J T, Urist M R, Caplan A I
Calcif Tissue Int. 1984 Sep;36(5):625-7. doi: 10.1007/BF02405378.
Urea and guanidine extracts of demineralized beef and rabbit bone matrix were assayed both in vivo and in vitro. One month following intramuscular implantation into mouse thighs, these extracts induced ectopic cartilage and bone. Seven days following continuous in vitro exposure to the same extracts, stage 24 chick limb bud mesenchymal cells in cultures had differentiated into greater numbers of chondrocytes than controls. These results suggest the feasibility of using limb bud mesenchymal cell cultures as an in vitro assay for bone matrix derived, extractable bioactive factors which effect the conversion of mesenchymal cells into chondrocytes as a requisite step in in vivo osteogenesis.
对脱矿质牛肉和兔骨基质的尿素和胍提取物进行了体内和体外测定。将这些提取物肌肉内植入小鼠大腿一个月后，诱导出了异位软骨和骨。在体外持续暴露于相同提取物七天后，培养中的24期鸡胚肢芽间充质细胞分化为软骨细胞的数量比对照组更多。这些结果表明，利用肢芽间充质细胞培养作为一种体外测定方法来检测骨基质衍生的、可提取的生物活性因子是可行的，这些因子影响间充质细胞向软骨细胞的转化，而这是体内骨生成的一个必要步骤。
