Harrison J H, Jollow D J
J Chromatogr. 1983 Oct 14;277:173-82. doi: 10.1016/s0378-4347(00)84834-3.
An assay method has been developed for the determination of the combined concentration of nitrosobenzene plus phenylhydroxylamine (as nitrosobenzene) in small volumes of blood. The initial step in the procedure consisted of the simultaneous oxidation of phenylhydroxylamine to nitrosobenzene and of ferrous hemoglobin to methemoglobin by ferricyanide. Nitrosobenzene in the ferricyanide-treated blood samples was then extracted into ethyl acetate, and separated and quantitated by reversed-phase high-performance liquid chromatography with electrochemical detection. The sensitivity limit for nitrosobenzene in blood was in the pmol/ml concentration range, less than 100 microliter of blood was required for assay, and the procedure was convenient for routine multisample use. In comparison with previous assays, this method was more sensitive, had a lower coefficient of variation, and required 25-40 fold smaller blood sample volumes. The method was combined with the orbital sinus bleeding technique in order to follow the nitrosobenzene time course in vivo using small serial blood samples from rats treated with intraperitoneal injections of phenylhydroxylamine or aniline.
已开发出一种测定小体积血液中亚硝基苯加苯胲（以亚硝基苯计）联合浓度的检测方法。该方法的第一步是通过铁氰化物将苯胲同时氧化为亚硝基苯，并将亚铁血红蛋白氧化为高铁血红蛋白。然后将经铁氰化物处理的血液样本中的亚硝基苯萃取到乙酸乙酯中，通过反相高效液相色谱法结合电化学检测进行分离和定量。血液中亚硝基苯的检测限在皮摩尔/毫升浓度范围内，检测所需血液量少于100微升，该方法便于常规多样本检测。与之前的检测方法相比，该方法更灵敏，变异系数更低，所需血液样本体积小25 - 40倍。该方法与眶窦出血技术相结合，以便使用经腹腔注射苯胲或苯胺处理的大鼠的小系列血液样本追踪体内亚硝基苯的时间进程。
