Hotz D, Brode E
J Chromatogr. 1983 Oct 14;277:217-27. doi: 10.1016/s0378-4347(00)84839-2.
A method with internal analogue standardization is described for the determination of 4-amino-6-methoxy-1-phenyl-pyridazinium methyl sulphate (ameziniummetilsulfate, Regulton, LU 1631) in plasma. The method involves a cation-exchange filtration, a dequaternization reaction and subsequent determination by high-performance liquid chromatography. There is no interference by plasma components or amezinium metabolites found in plasma extracts. When using a 2-ml sample the lower limit of detection is 0.75 ng/ml; the lower limit of determination is about 2 ng/ml. Interassay coefficients of variation decrease from about 14% at the lowest concentration to about 5% at 20 ng/ml and above.
描述了一种用于测定血浆中4-氨基-6-甲氧基-1-苯基哒嗪硫酸甲酯（阿美齐铵硫酸甲酯，Regulton，LU 1631）的内部模拟标准化方法。该方法包括阳离子交换过滤、去季铵化反应以及随后的高效液相色谱测定。血浆提取物中未发现血浆成分或阿美齐铵代谢物的干扰。使用2 ml样品时，检测下限为0.75 ng/ml；测定下限约为2 ng/ml。批间变异系数从最低浓度时的约14%降至20 ng/ml及以上时的约5%。
