26-羟基胆固醇：中国仓鼠卵巢细胞培养中羟甲基戊二酰辅酶A还原酶活性的调节
26-hydroxycholesterol: regulation of hydroxymethylglutaryl-CoA reductase activity in Chinese hamster ovary cell culture.
作者信息
Esterman A L, Baum H, Javitt N B, Darlington G J
出版信息
J Lipid Res. 1983 Oct;24(10):1304-9.
The effect of 26-hydroxycholesterol and other intermediates in bile acid synthesis on HMG-CoA reductase activity was studied in Chinese hamster ovary (CHO) cell culture. Incubation of CHO cells for 5 hr in 0.25 microM 26-hydroxycholesterol caused a 40% inhibition of HMG-CoA reductase activity. All other intermediates tested including 3 beta-hydroxy-5-cholenoic acid and cholest-5-ene-3 beta,7 alpha,26-triol, oxidation products of 26-hydroxycholesterol, had little or no inhibitory effect. It is proposed that 26-hydroxycholesterol has a selective biological role in the regulation of cholesterol synthesis.
在中国仓鼠卵巢（CHO）细胞培养中研究了26-羟基胆固醇及胆汁酸合成中的其他中间产物对HMG-CoA还原酶活性的影响。将CHO细胞在0.25微摩尔/升的26-羟基胆固醇中孵育5小时，可导致HMG-CoA还原酶活性受到40%的抑制。所测试的所有其他中间产物，包括3β-羟基-5-胆烯酸和胆甾-5-烯-3β,7α,26-三醇（26-羟基胆固醇的氧化产物），几乎没有或没有抑制作用。有人提出，26-羟基胆固醇在胆固醇合成的调节中具有选择性生物学作用。
Zotero 插件