Sinensky M, Torget R, Schnitzer-Polokoff R, Edwards P A
J Biol Chem. 1982 Jul 10;257(13):7284-6.
Regulation of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase activity and synthesis by 25-hydroxycholesterol is aberrant in a somatic cell mutant of the Chinese hamster ovary K1 cell auxotrophic for mevalonate by virtue of a defect in HMG-CoA synthase activity. Normal regulation of HMG-CoA reductase activity and synthesis in this mutant by 25-hydroxycholesterol can be restored by simultaneous incubation with a small (0.4 mM) mevalonate supplement. Normal regulation of HMG-CoA reductase is also observed in a revertant of the mutant cell with normal HMG-CoA synthase activity.
由于3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）合酶活性存在缺陷，中国仓鼠卵巢K1细胞营养缺陷型的体细胞突变体中，25-羟基胆固醇对HMG-CoA还原酶活性和合成的调节异常，该突变体因甲羟戊酸缺乏而表现出营养缺陷。通过与少量（0.4 mM）甲羟戊酸补充剂同时孵育，可以恢复该突变体中25-羟基胆固醇对HMG-CoA还原酶活性和合成的正常调节。在具有正常HMG-CoA合酶活性的突变体细胞回复株中，也观察到了HMG-CoA还原酶的正常调节。
