一种测定血液中UDP-半乳糖的新型大规模筛查方法。

A new mass screening method for determining UDP-galactose in blood.

作者信息

Fujimura Y, Kawamura M, Naruse H

出版信息

Tohoku J Exp Med. 1983 Nov;141(3):263-8. doi: 10.1620/tjem.141.263.

DOI:10.1620/tjem.141.263

Abstract

We devised a new microfluorometric mass screening method for determining UDP-galactose by using only one blood disc of 3 mm in diameter. This method contains two coupling enzymes of UDP-galactose-4-epimerase and UDP-glucose dehydrogenase, and conversion of NAD to NADH. The assay ranges are 0 to 1 mM or 0 to 0.2 mM UDP-galactose in blood. UDP-galactose contents were determined in galactosemia of epimerase-, transferase- and kinase-deficiencies, and several cases with high or low Paigen's values.

摘要

我们设计了一种新的微量荧光质量筛查方法，仅使用直径3毫米的一片血样来测定UDP-半乳糖。该方法包含UDP-半乳糖-4-表异构酶和UDP-葡萄糖脱氢酶这两种偶联酶，以及NAD向NADH的转化。血液中UDP-半乳糖的检测范围为0至1 mM或0至0.2 mM。我们测定了表异构酶、转移酶和激酶缺乏的半乳糖血症患者以及一些Paigen值高或低的病例的UDP-半乳糖含量。