结晶牛肝β-葡萄糖醛酸酶的制备。
Preparation of crystalline bovine liver beta-glucuronidase.
作者信息
Bonnichsen R, Heikkinen I
出版信息
Z Rechtsmed. 1978 May 29;81(2):97-102. doi: 10.1007/BF00203868.
PMID:664905
Abstract
A method is described for the preparation of pure beta-glucuronidase from bovine liver. The procedure includes ammonium sulfate, acetone and ethanol fractionation and a simple two-step ion exchange chromatography. The yield is acceptable and the method requires only standard laboratory equipment. The pure enzyme is easily crystallized from ammonium sulfate. Some practical applications of the pure beta-glucuronidase are discussed.
摘要
描述了一种从牛肝中制备纯β-葡萄糖醛酸酶的方法。该方法包括硫酸铵、丙酮和乙醇分级分离以及简单的两步离子交换色谱法。产率是可接受的，并且该方法仅需要标准实验室设备。纯酶很容易从硫酸铵中结晶出来。讨论了纯β-葡萄糖醛酸酶的一些实际应用。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Beta-Glucuronidase. II. Purification of calf liver beta-glucuronidase.β-葡萄糖醛酸酶。二、小牛肝脏β-葡萄糖醛酸酶的纯化。
J Biol Chem. 1953 Jun;202(2):763-9.
3
Purification and characterization of bovine liver beta-glucuronidase.牛肝β-葡萄糖醛酸酶的纯化与特性分析
Arch Biochem Biophys. 1966 Sep 26;116(1):193-206. doi: 10.1016/0003-9861(66)90027-0.
4
Demonstration and partial characterization of multiple forms of bovine liver beta-glucuronidase.牛肝β-葡萄糖醛酸酶多种形式的展示与部分特性分析
Biochemistry. 1967 Dec;6(12):3676-81. doi: 10.1021/bi00864a009.
5
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6
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