结晶牛肝β-葡萄糖醛酸酶的制备。
Preparation of crystalline bovine liver beta-glucuronidase.
作者信息
Bonnichsen R, Heikkinen I
出版信息
Z Rechtsmed. 1978 May 29;81(2):97-102. doi: 10.1007/BF00203868.
PMID:664905
Abstract
A method is described for the preparation of pure beta-glucuronidase from bovine liver. The procedure includes ammonium sulfate, acetone and ethanol fractionation and a simple two-step ion exchange chromatography. The yield is acceptable and the method requires only standard laboratory equipment. The pure enzyme is easily crystallized from ammonium sulfate. Some practical applications of the pure beta-glucuronidase are discussed.
摘要
描述了一种从牛肝中制备纯β-葡萄糖醛酸酶的方法。该方法包括硫酸铵、丙酮和乙醇分级分离以及简单的两步离子交换色谱法。产率是可接受的，并且该方法仅需要标准实验室设备。纯酶很容易从硫酸铵中结晶出来。讨论了纯β-葡萄糖醛酸酶的一些实际应用。
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