Starościk K, Waśniowska K, Syper D, Lisowska E
Arch Immunol Ther Exp (Warsz). 1983;31(2):127-33.
A simple and efficient method of purification of peanut agglutinin is described. This method involves affinity chromatography on human asialoglycophorin linked to Affi-Gel. One milliliter of the packed adsorbent contained 1.7 mg of covalently linked asialoglycophorin and bound about 6 mg of the peanut agglutinin. From 250 g of row peanuts 510 mg of the electrophoretically homogeneous lectin with high agglutinating activity was obtained.
本文描述了一种简单有效的花生凝集素纯化方法。该方法包括在与Affi-Gel相连的人去唾液酸糖蛋白上进行亲和层析。一毫升填充吸附剂含有1.7毫克共价连接的去唾液酸糖蛋白，可结合约6毫克花生凝集素。从250克生花生中获得了510毫克具有高凝集活性的电泳纯凝集素。
