Taskinen M R, Nikkilä E A, Kuusi T, Tulikoura I
Arteriosclerosis. 1983 Nov-Dec;3(6):607-15. doi: 10.1161/01.atv.3.6.607.
Serum lipoproteins were measured during a single infusion of intralipid and during parenteral nutrition with intralipid and glucose. Postheparin plasma lipolytic enzymes and plasma LCAT activity were assayed before and after the parenteral nutrition. Both single and repeated infusions of intralipid were followed by a significant rise of HDL2 concentration (p less than 0.01), whereas the HDL3 decreased. The composition of HDL subclasses altered. The HDL2 triglyceride and phospholipids increased, while the HDL3 esterified cholesterol and protein decreased. In vitro incubation of serum with intralipid alone caused no changes in the zonal profile of HDL subclasses, but hydrolysis of intralipid by lipoprotein lipase was followed by conversion of HDL3 into lighter particles floating in the density range of HDL2. The present results provide additional evidence for a precursor-product relationship between the HDL2 and HDL3. During 4 days of parenteral nutrition with intralipid, the basal (morning) values of serum total and VLDL triglyceride did not change. The LDL phospholipids increased progressively (from 67 to 98 mg/dl, p less than 0.05). The total HDL cholesterol decreased and this change was due to the fall of HDL3 cholesterol esters (from 19 to 12 mg/dl, p less than 0.05). Also the basal values of apo A-I and A-II in HDL3 decreased. The basal level of the HDL2 remained constant. Postheparin plasma LPL activity increased by 52% (p less than 0.01) but hepatic lipase activity fell by 49% (p less than 0.05). These changes may account for the maintenance of plasma HDL2, whereas the progressive fall of the basal HDL3 is probably due to the lack of intestinal apoprotein synthesis during absent intestinal absorption.
在单次输注脂肪乳剂期间以及使用脂肪乳剂和葡萄糖进行肠外营养期间，对血清脂蛋白进行了测量。在肠外营养前后，测定了肝素后血浆脂解酶和血浆卵磷脂胆固醇酰基转移酶（LCAT）活性。单次和重复输注脂肪乳剂后，高密度脂蛋白2（HDL2）浓度均显著升高（p<0.01），而高密度脂蛋白3（HDL3）降低。高密度脂蛋白亚类的组成发生了改变。HDL2的甘油三酯和磷脂增加，而HDL3的酯化胆固醇和蛋白质减少。血清仅与脂肪乳剂进行体外孵育不会导致HDL亚类的区带图谱发生变化，但脂蛋白脂肪酶对脂肪乳剂的水解会导致HDL3转化为漂浮在HDL2密度范围内的较轻颗粒。目前的结果为HDL2和HDL3之间的前体-产物关系提供了额外证据。在使用脂肪乳剂进行4天肠外营养期间，血清总甘油三酯和极低密度脂蛋白（VLDL）甘油三酯的基础（早晨）值没有变化。低密度脂蛋白（LDL）磷脂逐渐增加（从67mg/dl增至98mg/dl，p<0.05）。总HDL胆固醇降低，这种变化是由于HDL3胆固醇酯下降（从19mg/dl降至12mg/dl，p<0.05）。HDL3中载脂蛋白A-I和A-II的基础值也降低。HDL2的基础水平保持不变。肝素后血浆脂蛋白脂肪酶（LPL）活性增加了52%（p<0.01），但肝脂肪酶活性下降了49%（p<0.05）。这些变化可能解释了血浆HDL2的维持，而基础HDL3的逐渐下降可能是由于肠道吸收缺失期间肠道载脂蛋白合成不足所致。
