Paterson J R, Hood H T, Skellern G G
Biochem Biophys Res Commun. 1983 Oct 31;116(2):449-55. doi: 10.1016/0006-291x(83)90544-2.
The peroxidase and FAD-containing monooxygenase activities of porcine thyroid subcellular preparations were measured and it was observed that FAD-containing monooxygenase activity was considerably lower than that of peroxidase. The end product of 1-methyl-2[14C]thioimidazole oxidation catalysed by thyroid peroxidase was confirmed to be 1-methylimidazole by mass spectrometry. In the presence of thyroid peroxidase 1-methyl-2-thioimidazole would appear initially to be oxidised to bis(1-methylimidazole)-2,2'-disulphide. The extent of oxidation was dependent on the iodide concentration in the reaction mixture.
测定了猪甲状腺亚细胞制剂的过氧化物酶和含黄素腺嘌呤二核苷酸（FAD）的单加氧酶活性，观察到含FAD的单加氧酶活性明显低于过氧化物酶。通过质谱法确认，甲状腺过氧化物酶催化的1-甲基-2-[¹⁴C]硫代咪唑氧化的终产物为1-甲基咪唑。在甲状腺过氧化物酶存在的情况下，1-甲基-2-硫代咪唑最初似乎被氧化为双（1-甲基咪唑）-2,2'-二硫化物。氧化程度取决于反应混合物中的碘化物浓度。
