Kundu S K, Pleatman M A, Redwine W A, Boyd A E, Marcus D M
Biochem Biophys Res Commun. 1983 Nov 15;116(3):836-42. doi: 10.1016/s0006-291x(83)80218-6.
Monoclonal antibody A2B5 (Eisenbarth et al, Proc. Nat. Acad. Sci. (1979, 76:4913-4917), which reacts with neurons, thymic epithelium and peptide-hormone secreting cells of several species, was reported to react specifically with brain tetrasialogangliosides. We have found that A2B5 binds to gangliosides GQ1b, GD3, GD2, disialolactoneotetraosylceramide, and probably to GT1a, when assayed by an immunostaining procedure that detects binding of antibody to gangliosides on a thin-layer plate. Additional data obtained by complement fixation revealed that this antibody reacted most strongly with ganglioside GQ1b almost as well with disialogangliosides GD3, GD2 and disialolactoneotetraosylceramide, weakly with GD1b and GT1b, and very weakly with GM3 and GD1a. These data indicate that A2B5 cannot be regarded as a specific reagent for the recognition of tetrasialogangliosides.
单克隆抗体A2B5（艾森巴思等人，《美国国家科学院院刊》（1979年，76:4913 - 4917））可与多种物种的神经元、胸腺上皮细胞以及肽激素分泌细胞发生反应，据报道它能与脑四唾液酸神经节苷脂特异性反应。我们发现，通过一种免疫染色程序检测抗体与薄层板上神经节苷脂的结合情况时，A2B5可与神经节苷脂GQ1b、GD3、GD2、二唾液酸乳糖四糖神经酰胺结合，可能还与GT1a结合。通过补体结合获得的其他数据表明，该抗体与神经节苷脂GQ1b反应最强，与二唾液酸神经节苷脂GD3、GD2和二唾液酸乳糖四糖神经酰胺反应程度相近，与GD1b和GT1b反应较弱，与GM3和GD1a反应极弱。这些数据表明，A2B5不能被视为识别四唾液酸神经节苷脂的特异性试剂。
