DeSimone D P, Reddi A H
Calcif Tissue Int. 1983 Sep;35(6):732-9. doi: 10.1007/BF02405115.
The influence of retinoic acid on matrix-induced endochondral bone differentiation was determined. Retinoic acid was administered during discrete stages of endochondral bone formation, specifically, mesenchymal cell proliferation, chondrogenesis, bone formation, and mineralization. In retinoic acid-treated rats examined on day 3 following matrix implantation, biochemical markers for mesenchymal cell proliferation were about 50% of the controls. Chondrogenesis on day 7, assessed by 35 SO4 incorporation into proteoglycans, was 27% of the control. In addition, dissociative extraction of proteoglycans with 4.0 M guanidine-HCl and chromatography on Sepharose CL-2B revealed the synthesis of a smaller molecular weight proteoglycan when compared to controls which exhibited the cartilage-specific type. Osteogenesis and bone mineralization were monitored by alkaline phosphatase activity and 45Ca incorporation. On day 11 alkaline phosphatase activity was decreased by 40% and 45Ca incorporation was 48% of the control. These results revealed the multiple foci of the actions of excess vitamin A.
确定了视黄酸对基质诱导的软骨内骨分化的影响。在软骨内骨形成的不同阶段给予视黄酸，具体为间充质细胞增殖、软骨形成、骨形成和矿化阶段。在基质植入后第3天检查的视黄酸处理大鼠中，间充质细胞增殖的生化标志物约为对照组的50%。通过将35SO4掺入蛋白聚糖评估的第7天软骨形成，为对照组的27%。此外，用4.0M盐酸胍对蛋白聚糖进行解离提取并在琼脂糖CL-2B上进行色谱分析，结果显示与表现出软骨特异性类型的对照组相比，合成了分子量较小的蛋白聚糖。通过碱性磷酸酶活性和45Ca掺入监测成骨和骨矿化。在第11天，碱性磷酸酶活性降低了40%，45Ca掺入为对照组的48%。这些结果揭示了过量维生素A作用的多个靶点。
