Calarco-Gillam P D, Siebert M C, Hubble R, Mitchison T, Kirschner M
Cell. 1983 Dec;35(3 Pt 2):621-9. doi: 10.1016/0092-8674(83)90094-6.
A human autoantibody from a schleroderma patient was found to immunostain interphase and mitotic centrosomes in a variety of vertebrate cells. Electron microscopic immunocytochemistry localized this antigen in dense pericentriolar material (PCM) surrounding the centrioles. The meiotic spindle of the mouse egg has no centriole but it exhibited a broad PCM band at each pole. This pattern was also found from the first through fourth mitotic divisions. During this time PCM was found assembled at a single locus in the cell and exclusively in mitotic cells; it was not observable in interphase cells. In the blastocyst, only polar trophoblast cells had characteristic centrosomes throughout the cell cycle. Results suggest PCM can exist, disperse, and reorganize during the cell cycle independently of the centriole, and its distribution in the embryo differs in cells having different fates.
从一名硬皮病患者体内发现的一种人类自身抗体，能对多种脊椎动物细胞的间期和有丝分裂中心体进行免疫染色。电子显微镜免疫细胞化学将这种抗原定位在围绕中心粒的致密中心粒周围物质（PCM）中。小鼠卵细胞的减数分裂纺锤体没有中心粒，但在每个极都呈现出一条宽阔的PCM带。从第一次到第四次有丝分裂也发现了这种模式。在此期间，PCM在细胞中的单个位点组装，且仅存在于有丝分裂细胞中；在间期细胞中无法观察到。在囊胚中，只有极性滋养层细胞在整个细胞周期中具有特征性的中心体。结果表明，PCM可以在细胞周期中独立于中心粒存在、分散和重新组织，并且其在胚胎中的分布在具有不同命运的细胞中有所不同。
