Sikerwar S S, Malhotra S K
Cell Biol Int Rep. 1983 Nov;7(11):897-903. doi: 10.1016/0309-1651(83)90208-4.
Mouse liver gap junctions have been isolated by using an anionic detergent, n-dodecanoyl sarcosine, in combination with non-ionic polyoxyethylene ethers (Brij 35 and Brij 58) and (W-1) detergents. Purified gap junctions are obtained in a sucrose step gradient containing 1-o-n-octyl-beta-D-glucopyranoside. This procedure is aimed at minimizing proteolysis. The junctions thus isolated have a hexagonal lattice of connexons with a lattice constant of 7.6-8.4 nm. Presence of a major Mr 26,000 gap junctional protein has been confirmed by SDS-PAGE.
通过使用阴离子去污剂n-十二烷酰肌氨酸，并结合非离子聚氧乙烯醚（Brij 35和Brij 58）以及（W-1）去污剂，从小鼠肝脏中分离出了间隙连接。在含有1-正辛基-β-D-吡喃葡萄糖苷的蔗糖阶梯梯度中获得了纯化的间隙连接。该程序旨在最大程度地减少蛋白水解。如此分离出的连接具有连接子的六边形晶格，晶格常数为7.6 - 8.4纳米。通过SDS-PAGE证实了主要的26,000道尔顿间隙连接蛋白的存在。
