Margulies M M
Eur J Biochem. 1983 Dec 1;137(1-2):241-8. doi: 10.1111/j.1432-1033.1983.tb07821.x.
Messenger RNA activities associated with thylakoids of Chlamydomonas reinhardtii were examined by translation of RNA of rough thylakoids (thylakoids with bound ribosomes) in a wheat germ protein-synthesis system. The RNA fraction, active in translation, did not bind to oligod (T)-cellulose indicating that the mRNA associated with the rough thylakoids contains little, if any, 3' polyadenylic acid. Assuming that 1% of the thylakoid RNA was mRNA, it was found to have a specific translation activity between that of globin mRNA and tobacco mosaic virus RNA. Translation products of thylakoid RNA were immunoprecipitated by crossed immunoelectrophoresis, using antisera to two polypeptides synthesized within the chloroplast, one of which specifically precipitates the beta subunit of chloroplast coupling factor 1. Each antiserum immunoprecipitated radioactive polypeptide from translates of thylakoid RNA, indicating that the presence of mRNA for both polypeptides is associated with the thylakoids. The results suggest that thylakoid-bound polyribosomes are involved in the synthesis of thylakoid polypeptides.
通过在小麦胚芽蛋白质合成系统中翻译粗糙类囊体（带有结合核糖体的类囊体）的RNA，对莱茵衣藻类囊体相关的信使RNA活性进行了检测。在翻译中具有活性的RNA组分不与寡聚（T）纤维素结合，这表明与粗糙类囊体相关的信使RNA即使含有3'聚腺苷酸，含量也很少。假设类囊体RNA的1%是信使RNA，发现其比活性介于珠蛋白信使RNA和烟草花叶病毒RNA之间。使用针对叶绿体中合成的两种多肽的抗血清，通过交叉免疫电泳对类囊体RNA的翻译产物进行免疫沉淀，其中一种抗血清能特异性沉淀叶绿体偶联因子1的β亚基。每种抗血清都能从类囊体RNA的翻译产物中免疫沉淀出放射性多肽，这表明两种多肽的信使RNA都与类囊体相关。结果表明，与类囊体结合的多核糖体参与了类囊体多肽的合成。
