Rognstad R
Int J Biochem. 1983;15(12):1417-21. doi: 10.1016/0020-711x(83)90073-3.
At low L-lactate concentrations, plots of the reciprocal of the gluconeogenic rate vs hydroxycyanocinnamate concentration were linear up to high inhibitor concentrations, indicating that the pyruvate transporter was a rate-limiting step and alternate pathways were at best minor. At 10 mM L-lactate and in the absence of added acids, the 1/V vs I plots became sigmoidal, indicating both some excess capacity of the transporter, and a significant alternative pathway. Use of transaminase inhibitors suggests that the alternate pathway does not primarily involve a dual glutamate-pyruvate transaminase mechanism.
在低L-乳酸浓度下，糖异生速率的倒数与羟基氰基肉桂酸酯浓度的关系图在高抑制剂浓度之前呈线性，这表明丙酮酸转运体是限速步骤，替代途径充其量只是次要的。在10 mM L-乳酸且不添加酸的情况下，1/V与I的关系图呈S形，这表明转运体有一些过剩能力，且存在一条重要的替代途径。使用转氨酶抑制剂表明，替代途径主要不涉及双谷氨酸-丙酮酸转氨酶机制。
