Chu L L, MacGregor R R, Hamilton J W
Mol Cell Endocrinol. 1983 Dec;33(2-3):157-68. doi: 10.1016/0303-7207(83)90164-8.
Tissue slices or dispersed cells of bovine parathyroid gland were incubated with [3H]leucine to label the intracellular proteins and then tested for their secretory response to isoproterenol and cycloheximide at different calcium concentrations. Secretion of the newly synthesized as well as the older PTH and SP-I was stimulated by isoproterenol at all calcium levels tested, even when it was maximally enhanced by low calcium. Cycloheximide interfered with neither the secretory process nor the secretory response to different stimuli, but decreased the amount of PTH and SP-I secreted. We conclude that the inhibitor decreased the secretion by reducing the supply of PTH and SP-I. Calculations derived from the data reveal that, under most secretory conditions, newly synthesized PTH contributed a major portion of the total hormone secretion in bovine parathyroid cells.
将牛甲状旁腺的组织切片或分散细胞与[3H]亮氨酸一起孵育，以标记细胞内蛋白质，然后在不同钙浓度下测试它们对异丙肾上腺素和环己酰亚胺的分泌反应。在所测试的所有钙水平下，异丙肾上腺素均能刺激新合成的以及较老的甲状旁腺激素（PTH）和甲状旁腺分泌蛋白-I（SP-I）的分泌，即使在低钙使其分泌最大化增强时也是如此。环己酰亚胺既不干扰分泌过程，也不干扰对不同刺激的分泌反应，但会减少分泌的PTH和SP-I的量。我们得出结论，该抑制剂通过减少PTH和SP-I的供应来降低分泌。根据数据进行的计算表明，在大多数分泌条件下，新合成的PTH在牛甲状旁腺细胞的总激素分泌中占主要部分。
