Neurath A R, Strick N, Baker L, Krugman S
Dev Biol Stand. 1983;54:143-50.
Antigens corresponding to infectious agents may be present in biological specimens only in a cryptic form bound to antibodies and thus may elude detection. We describe a solid-phase technique for separation of antigens from antibodies. Immune complexes are precipitated from serum by polyethylene glycol, dissociated with NaSCN and adsorbed onto nitrocellulose or polystyrene supports. Antigens remain topographically separated from antibodies after removal of NaSCN and can be detected with radiolabeled antibodies. Genomes from viruses immobilized on nitrocellulose can be identified by nucleic acid hybridization. Nanogram quantities of sequestered hepatitis B surface and core antigens and picogram amounts of hepatitis B virus DNA are detected.
与感染因子相对应的抗原可能仅以与抗体结合的隐蔽形式存在于生物标本中，因此可能无法被检测到。我们描述了一种从抗体中分离抗原的固相技术。免疫复合物通过聚乙二醇从血清中沉淀出来，用硫氰酸钠解离并吸附到硝酸纤维素或聚苯乙烯载体上。去除硫氰酸钠后，抗原在地形上与抗体分离，可用放射性标记抗体进行检测。固定在硝酸纤维素上的病毒基因组可通过核酸杂交进行鉴定。检测到纳克量的隐匿性乙型肝炎表面和核心抗原以及皮克量的乙型肝炎病毒DNA。
