Nikodem V M, Huang D R, Trus B L, Rall J E
Horm Metab Res. 1983 Nov;15(11):550-4. doi: 10.1055/s-2007-1018785.
The effect of thyroid hormone on acetylation, phosphorylation and ADP ribosylation of rat liver nucleoproteins was studied by incubating intact nuclei with labeled precursors. Acetylation, which occurred in histones and low molecular weight proteins (less than 30,000), was depressed in nuclei from thyroidectomized animals. The administration of L-3,5,3'-triiodothyronine (T3) increased acetate incorporation to 50% over control levels. Incorporation of labeled phosphate from ATP into most proteins was decreased in nuclei from thyroidectomized animals and increased by the administration of T3. The greatest increase produced by T3 (to 140% of control values) was seen in proteins of molecular weight greater than 68,000. Nuclei from thyroidectomized animals incorporated less ADP ribose in most proteins. Both high molecular weight proteins (greater than 68,000) and low molecular weight proteins (less than 30,000) showed a further decrease in ADP ribose incorporation in nuclei from thyroidectomized rats given T3. However, a few proteins of the middle molecular weight class showed increased ADP ribose incorporation subsequent to the injection of T3. It is suggested that a generalized increase in protein synthetic rates previously noted to be caused by T3 is accompanied by increased acetylation and phosphorylation of histones and other proteins. These changes could accelerate transcription of already active genes.
通过用标记前体孵育完整细胞核，研究了甲状腺激素对大鼠肝脏核蛋白乙酰化、磷酸化和ADP核糖基化的影响。乙酰化发生在组蛋白和低分子量蛋白（小于30,000）中，甲状腺切除动物的细胞核中乙酰化受到抑制。给予L-3,5,3'-三碘甲状腺原氨酸（T3）后，乙酸掺入量比对照水平增加了50%。甲状腺切除动物的细胞核中，ATP中标记磷酸盐掺入大多数蛋白的量减少，而给予T3后则增加。T3产生的最大增加量（达到对照值的140%）出现在分子量大于68,000的蛋白中。甲状腺切除动物的细胞核在大多数蛋白中掺入的ADP核糖较少。高分子量蛋白（大于68,000）和低分子量蛋白（小于30,000）在给予T3的甲状腺切除大鼠的细胞核中，ADP核糖掺入量都进一步减少。然而，少数中等分子量类别的蛋白在注射T3后显示出ADP核糖掺入增加。有人提出，先前注意到由T3引起的蛋白质合成速率普遍增加伴随着组蛋白和其他蛋白乙酰化和磷酸化的增加。这些变化可能会加速已激活基因的转录。
