Matkowitz R, Junghans P, Jung K, Hartig W, Faust H
Infusionsther Klin Ernahr. 1983 Oct;10(5):268-75.
A standardized 15N tracer method is described for the assessment of the nitrogen and protein metabolism in healthy and pathological changed organism. The method represents an isotope technical procedure for the application in the clinical research and practice. The single parts of this routine method are: --the clinical preparation of the patient/proband by means of a standardized nutritional regime, --the tracer administration (single dose) and the sampling (urine, blood), --the 15N-tracer technique (sample chemistry, emissions-spectrometric isotope analysis), --the mathematical evaluation of 15N tracer data.
描述了一种标准化的15N示踪方法,用于评估健康和病理变化机体中的氮和蛋白质代谢。该方法是一种应用于临床研究和实践的同位素技术程序。该常规方法的各个部分包括:——通过标准化营养方案对患者/受试者进行临床准备,——示踪剂给药(单次剂量)和采样(尿液、血液),——15N示踪技术(样品化学、发射光谱同位素分析),——15N示踪数据的数学评估。