Asaoka K, Takahashi K
J Biochem. 1983 Nov;94(5):1685-8.
Conditions have been examined for the use of o-dinitrobenzene as a substrate for colorimetric assay of glutathione S-transferases. Activities can be determined by measuring nitrite released enzymatically from the substrate using a diazo-coupling method with N-(1-naphthyl)ethylenediamine dihydrochloride and sulfanilamide. The assay can be done in the presence of large amounts of reduced glutathione (GSH), cysteine, and protein, and is capable of quantitating the enzyme activity in about 30 micrograms (wet weight) of monkey liver, corresponding to 0.1 microgram of purified glutathione S-transferase from the same source. This method is suitable for assaying simultaneously a large number of samples with reasonable sensitivity and speed.
已对使用邻二硝基苯作为谷胱甘肽S-转移酶比色测定底物的条件进行了研究。活性可通过使用N-（1-萘基）乙二胺二盐酸盐和磺胺的重氮偶联法测量从底物酶促释放的亚硝酸盐来确定。该测定可在大量还原型谷胱甘肽（GSH）、半胱氨酸和蛋白质存在的情况下进行，并且能够定量约30微克（湿重）猴肝中的酶活性，相当于来自同一来源的0.1微克纯化谷胱甘肽S-转移酶。该方法适用于以合理的灵敏度和速度同时检测大量样品。
