Immunologic detection of specific proteins in cell extracts by fractionation in gels and transfer to paper.
作者信息
Reiser J, Stark G R
出版信息
Methods Enzymol. 1983;96:205-15. doi: 10.1016/s0076-6879(83)96018-4.
PMID:6656630
Abstract
摘要
相似文献
1
Immunologic detection of specific proteins in cell extracts by fractionation in gels and transfer to paper.通过凝胶分级分离和转印至纸张对细胞提取物中的特定蛋白质进行免疫学检测。
Methods Enzymol. 1983;96:205-15. doi: 10.1016/s0076-6879(83)96018-4.
2
Immunological detection of specific proteins in total cell extracts by fractionation in gels and transfer to diazophenylthioether paper.通过凝胶分级分离和转移至重氮苯硫醚纸上，对全细胞提取物中的特定蛋白质进行免疫检测。
Eur J Biochem. 1981 Mar;114(3):569-75. doi: 10.1111/j.1432-1033.1981.tb05182.x.
3
Immunochemical identification of membrane proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后膜蛋白的免疫化学鉴定
Methods Enzymol. 1983;96:192-205. doi: 10.1016/s0076-6879(83)96017-2.
4
Direct identification of specific glycoproteins and antigens in sodium dodecyl sulfate gels.十二烷基硫酸钠凝胶中特定糖蛋白和抗原的直接鉴定
Methods Enzymol. 1978;50:54-64. doi: 10.1016/0076-6879(78)50007-4.
5
Crossed immunoelectrophoresis from sodium dodecyl sulfate- polyacrylamide gels after fixation and staining and without nonionic detergent intermediate layers.固定、染色后且无非离子去污剂中间层的十二烷基硫酸钠-聚丙烯酰胺凝胶交叉免疫电泳。
Anal Biochem. 1981 Sep 1;116(1):129-32. doi: 10.1016/0003-2697(81)90333-x.
6
Two 2-dimensional electrophoreses: a combination of crossed immunoelectrophoresis and dodecyl sulfate polyacrylamide gel electrophoresis for protein analysis.两种二维电泳：交叉免疫电泳与十二烷基硫酸钠聚丙烯酰胺凝胶电泳相结合用于蛋白质分析。
Experientia. 1980 Jul 15;36(7):816-7. doi: 10.1007/BF01978588.
7
SDS polyacrylamide gel electrophoresis of proteins.蛋白质的十二烷基硫酸钠聚丙烯酰胺凝胶电泳
Methods Mol Biol. 1994;32:23-34. doi: 10.1385/0-89603-268-X:23.
8
Antigen detection in immune complexes by a modified staphylococci binding assay and Western blot analysis.
Clin Immunol Immunopathol. 1986 Feb;38(2):184-97. doi: 10.1016/0090-1229(86)90137-6.
9
Western blotting and immunochemical detection of histones electrophoretically resolved on acid-urea-triton- and sodium dodecyl sulfate-polyacrylamide gels.在酸性尿素- Triton和十二烷基硫酸钠-聚丙烯酰胺凝胶上电泳分离的组蛋白的蛋白质免疫印迹法和免疫化学检测。
Anal Biochem. 1992 Feb 1;200(2):339-41. doi: 10.1016/0003-2697(92)90475-m.
10
Continuous Elution SDS-PAGE with a Modified Standard Gel Apparatus to Separate and Isolate an Array of Proteins from Complex Mixtures.使用改良标准凝胶装置进行连续洗脱SDS-PAGE以从复杂混合物中分离和分离一系列蛋白质。
Methods Mol Biol. 2019;1855:467-478. doi: 10.1007/978-1-4939-8793-1_39.
引用本文的文献
1
Molecular analysis of the Trichosporon cutaneum DSM 70698 argA gene and its use for DNA-mediated transformations.皮状丝孢酵母DSM 70698精氨酸琥珀酸合成酶基因的分子分析及其在DNA介导转化中的应用。
J Bacteriol. 1994 May;176(10):3021-32. doi: 10.1128/jb.176.10.3021-3032.1994.
2
Cloning and heterologous expression of a gene encoding an alkane-induced extracellular protein involved in alkane assimilation from Pseudomonas aeruginosa.克隆并异源表达来自铜绿假单胞菌的一个编码参与烷烃同化的烷烃诱导细胞外蛋白的基因。
Appl Environ Microbiol. 1994 Oct;60(10):3679-87. doi: 10.1128/aem.60.10.3679-3687.1994.
3
Bordetella pertussis adenylate cyclase: isolation and purification by calmodulin-sepharose 4B chromatography.百日咳博德特氏菌腺苷酸环化酶：通过钙调蛋白琼脂糖4B层析法进行分离与纯化
Infect Immun. 1987 Jan;55(1):129-34. doi: 10.1128/iai.55.1.129-134.1987.
Zotero 插件