Lee T Q, Schmid-Schönbein G W, Zweifach B W
Microvasc Res. 1983 Nov;26(3):351-61. doi: 10.1016/0026-2862(83)90084-5.
The dual-window photometric analyzer is currently a widely used instrument to estimate volumetric flow rates in microvessels. This instrument provides an analog voltage that is proportional to cell velocity, but the question arises how this voltage is related to the mean velocity in the blood vessel. It requires a separate investigation and in this report a calibration of an improved version of a dual-window photometric analyzer for volumetric flow rate measurements in microvessels as large as 470 microns in diameter is presented. The relationship between correlator velocity and blood mean velocity is presented in a closed empirical form. Within a 5% error of the mean it is found to be dependent on tube diameter but independent of hematocrit (between 3 and 40%). A rotating transparent disc plated with dried red blood cells was used to test the dynamic response of the instrument for frequencies up to 10 Hz. The amplitude reduction was less than 10% up to 3 Hz. Thereafter it fell off at 6 db/octave.
双窗口光度分析仪是目前广泛用于估计微血管容积流速的仪器。该仪器提供与细胞速度成比例的模拟电压，但问题是该电压与血管中的平均速度有何关系。这需要单独研究，本报告介绍了一种改进版双窗口光度分析仪的校准情况，该校准用于测量直径达470微米的微血管中的容积流速。相关器速度与血液平均速度之间的关系以封闭的经验形式给出。在平均误差5%范围内，发现其取决于管径，但与血细胞比容（3%至40%之间）无关。使用镀有干燥红细胞的旋转透明圆盘测试该仪器在高达10 Hz频率下的动态响应。在高达3 Hz时，幅度降低小于10%。此后，它以6 dB/倍频程下降。
