DeLisle A J, Graves R A, Marzluff W F, Johnson L F
Mol Cell Biol. 1983 Nov;3(11):1920-9. doi: 10.1128/mcb.3.11.1920-1929.1983.
We measured the content and metabolism of histone mRNA in mouse 3T6 fibroblasts during a serum-induced transition from the resting to growing state. The content of several histone H3 and H2b mRNAs was measured by an S1 nuclease procedure. All of these increase in parallel by a factor of about 50 during S phase. However, the rate of H3 gene transcription increased only fivefold during this period, as determined in an in vitro transcription assay. This suggests that histone mRNA content is also controlled at the posttranscriptional level. When resting cells were serum stimulated in the presence of cytosine arabinoside, the rate of H3 gene transcription increased to about the same extent as that in control-stimulated cells. However, cytoplasmic H3 mRNA content increased only five to seven-fold. The half-life of H3 mRNA during S phase was about 4 to 5 h. When cytosine arabinoside was added to cells in the S phase, the half-life of the message decreased to about 15 min. The rapid turnover of H3 mRNA was prevented when the drug was added in the presence of cycloheximide or puromycin. The rate of H3 gene transcription decreased by only 35% after treatment with cytosine arabinoside. These results suggest that H3 gene transcription is not tightly coupled to DNA replication but is controlled temporally during the resting to growing transition. However, there is a correlation between the rate of DNA synthesis and the stability of histone H3 mRNA.
我们检测了血清诱导小鼠3T6成纤维细胞从静止状态转变为生长状态过程中组蛋白mRNA的含量及代谢情况。通过S1核酸酶法检测了几种组蛋白H3和H2b mRNA的含量。在S期，所有这些mRNA的含量均平行增加约50倍。然而，如体外转录试验所测定的，在此期间H3基因转录速率仅增加了5倍。这表明组蛋白mRNA含量在转录后水平也受到调控。当静止细胞在阿糖胞苷存在的情况下受到血清刺激时，H3基因转录速率增加的幅度与对照刺激细胞大致相同。然而，细胞质中H3 mRNA含量仅增加了5至7倍。S期H3 mRNA的半衰期约为4至5小时。当在S期向细胞中加入阿糖胞苷时，该信使RNA的半衰期降至约15分钟。当在放线菌酮或嘌呤霉素存在的情况下加入该药物时，H3 mRNA的快速周转被阻止。用阿糖胞苷处理后，H3基因转录速率仅下降了35%。这些结果表明，H3基因转录与DNA复制并非紧密偶联，而是在从静止到生长的转变过程中受到时间上的调控。然而，DNA合成速率与组蛋白H3 mRNA的稳定性之间存在相关性。
