Bremmelgaard A
Scand J Clin Lab Invest. 1983 Nov;43(7):603-7.
Bile acids were measured in plasma of three non-fasting bilaterally nephrectomized patients in chronic haemodialysis. After separation of the bile acid mixture according to mode of conjugation and cleavage of amide and sulphate ester bonds, the bile acids were analysed as their methylester trimethylsilyl ethers on gas-liquid chromatography-mass spectrometry (GC-MS). The total bile acid concentration was 4.10-13.70 mumol X l(-1) (N: less than 14 mumol X l(-1)) mostly glycine conjugates. Unconjugated bile acids were found in significant amounts in one patient and in trace amounts in another patient. Chenodeoxycholic acid was the predominant individual bile acid constituting 1.89-6.79 mumol X l(-1) (N: less than 10 mumol X l(-1)). The oxidoreduction product of this bile acid: ursodeoxycholic acid, was found in all patients, whereas the 7 alpha-dehydroxylation product: lithocholic acid, was absent. 1 beta-hydroxy-deoxycholic acid was a major constituent in two of the cases. Hyocholic acid, the 6 alpha-hydroxylation product of chenodeoxycholic acid was detected in two cases. Tetrahydroxy bile acids and sulphated bile acids were not found in significant amounts.
对三名接受慢性血液透析的双侧肾切除非空腹患者的血浆胆汁酸进行了测量。根据结合方式以及酰胺键和硫酸酯键的裂解分离胆汁酸混合物后，将胆汁酸作为其甲酯三甲基硅醚通过气-液色谱-质谱联用仪（GC-MS）进行分析。总胆汁酸浓度为4.10 - 13.70μmol·L⁻¹（正常范围：小于14μmol·L⁻¹），主要为甘氨酸结合物。在一名患者中发现了大量未结合胆汁酸，在另一名患者中发现了微量未结合胆汁酸。鹅去氧胆酸是主要的单一胆汁酸，浓度为1.89 - 6.79μmol·L⁻¹（正常范围：小于10μmol·L⁻¹）。该胆汁酸的氧化还原产物：熊去氧胆酸，在所有患者中均有发现，而7α-脱羟基产物：石胆酸，则未检出。1β-羟基脱氧胆酸在两例中是主要成分。鹅胆酸，即鹅去氧胆酸的6α-羟基化产物，在两例中被检测到。未发现大量的四羟基胆汁酸和硫酸化胆汁酸。
