Dhaliwal J S, Lim T W, Sukumaran K D
Southeast Asian J Trop Med Public Health. 1983 Sep;14(3):367-73.
A micro ELISA assay was established to diagnose systemic poisoning for the rapid administration of specific antivenom. Rabbit anti venom IgG was bound to the solid phase to enable detection of venom from both the Malayan Pit Viper (Agkistrodon rhodostoma) and the Common Cobra (Naja naja). This assay is read visually and takes 35 to 45 minutes to perform. It can detect 15.6 ng/ml of viper venom in 75 minutes and 7.8 ng/ml of cobra venom in 55 minutes. Tests on sera from snake bite patients showed detectable levels of snake venom in the serum even though administration of antivenom was not necessary. Furthermore, results from these clinical cases were obtained in less than 45 minutes. It was found that the most suitable washing media was saline/Tween, the assay could be performed at room temperature and plates stored for 6 months showed no loss of activity.
建立了一种微量酶联免疫吸附测定法，用于诊断全身性中毒，以便快速施用特定抗蛇毒血清。将兔抗蛇毒IgG固定在固相上，以检测马来亚蝮蛇（红口蝮）和眼镜蛇（眼镜蛇）的毒液。该测定法通过目视读取，操作需要35至45分钟。它可以在75分钟内检测到15.6 ng/ml的蝰蛇毒液，在55分钟内检测到7.8 ng/ml的眼镜蛇毒液。对蛇咬伤患者血清的检测表明，即使不需要施用抗蛇毒血清，血清中也能检测到蛇毒水平。此外，这些临床病例的结果在不到45分钟内即可获得。发现最合适的洗涤介质是盐水/吐温，该测定法可在室温下进行，储存6个月的平板未显示活性丧失。
