Kuwano M, Masuda A, Hidaka K, Akiyama S I
Somatic Cell Genet. 1983 Nov;9(6):659-72. doi: 10.1007/BF01539471.
Amphotericin B-resistant mutants isolated from Chinese hamster V79 cells (1) are defective in cholesterol synthesis and more sensitive to an oxygenated sterol analog, 7-ketocholesterol, than their parental cell line. We isolated 7-ketocholesterol-resistant mutants from an amphotericin B-resistant mutant, AMBR-1. The 7-ketocholesterol-resistant mutants had regained increased level of free cholesterol, and they showed somewhat similar dose-response curves to amphotericin B as that of V79. Sterol synthesis from acetate, but not from mevalonate, in 7-ketocholesterol-resistant clones was threefold higher than that of AMBR-1. 7-Ketocholesterol-resistant clone, unlike AMBR-1, could form colonies in the presence of lipoprotein-depleted serum. The results are discussed in terms of probable change in the sterol biosynthetic pathway by the different lesions.
从中国仓鼠V79细胞中分离出的对两性霉素B耐药的突变体（1）在胆固醇合成方面存在缺陷，并且比其亲本细胞系对氧化甾醇类似物7-酮胆固醇更敏感。我们从两性霉素B耐药突变体AMBR-1中分离出了对7-酮胆固醇耐药的突变体。对7-酮胆固醇耐药的突变体恢复了较高水平的游离胆固醇，并且它们对两性霉素B的剂量反应曲线与V79细胞的有些相似。在对7-酮胆固醇耐药的克隆中，由乙酸盐而非甲羟戊酸合成甾醇的能力比AMBR-1高两倍。与AMBR-1不同，对7-酮胆固醇耐药的克隆在缺乏脂蛋白的血清存在下能够形成集落。根据不同损伤对甾醇生物合成途径可能产生的变化对结果进行了讨论。
