Baruffi F, De Tata V, Gori Z
Boll Soc Ital Biol Sper. 1983 Oct 30;59(10):1412-5.
The changes in hexokinase (HK), glucose-6-phosphate dehydrogenase (G6P-DH) and glutamate aspertate aminotransferase (GOT) activities with the location of tissue within the left ventricle wall have been explored in the rat myocardium. The hearts were cut in 100 micron thick serial sections (see 4) and all sections spectrophotometric procedures (5). No significant transmural gradient in HK activity was observed but the levels of G6P-DH and of GOT activities were significantly higher in the subepicardial tissue and were at their lowest levels in the midmyocardial layers. Our data and previous observations (3,6) indicate that adptions to regional differences in the cardiac work load occurred in the left ventricle wall but that the transmural patterns of enzyme distribution may change with the different animal specie.
在大鼠心肌中，研究了己糖激酶（HK）、葡萄糖-6-磷酸脱氢酶（G6P-DH）和谷氨酸草酰乙酸转氨酶（GOT）活性随左心室壁内组织位置的变化。将心脏切成100微米厚的连续切片（见图4），并对所有切片进行分光光度法检测（见图5）。未观察到HK活性有明显的透壁梯度，但G6P-DH和GOT活性水平在心外膜下组织中显著较高，而在心肌中层处于最低水平。我们的数据和先前的观察结果（3,6）表明，左心室壁会适应心脏工作负荷的区域差异，但酶分布的透壁模式可能会因动物种类不同而改变。
