Johnson T L, Knowles C O
Gen Pharmacol. 1983;14(6):591-6. doi: 10.1016/0306-3623(83)90154-4.
Biogenic amine levels in samples of whole brain and plasma following treatment of rats with chlordimeform (CDF), its two N-demethyl metabolites (DMC and DDC), p-chloroamphetamine (PCA), and harmaline were separated by high performance liquid chromatography equipped with fluorescence or electrochemical detection systems. At 1 hr following s.c. injection, CDF (200 mg/kg) caused a reduction in levels of norepinephrine (NE), 5-hydroxytryptamine (5-HT), and tyramine (TRM), an increase in dopamine (DA), and no change in levels of beta-phenethylamine (PEA) in samples of whole rat brain, whereas DMC (100 mg/kg) and DDC (25 mg/kg) effected reductions of brain levels of NE, 5-HT, TRM, and PEA with no change in DA. The effect of DMC (100 mg/kg) on NE and DA levels in brain was followed periodically for 24 hr. Following a significant decrease at 1 hr, NE levels increased to a maximum at 12 hr and remained higher than controls throughout the remainder of the 24 hr test period. DA levels increased during the initial 12 hr and remained significantly higher than controls for the remaining 12 hr. The influence of s.c. vs i.p. administration of DMC (100 mg/kg) on brain amine levels was examined. Intraperitoneal treatment generally resulted in lower amine levels in DMC and vehicle treated animals. Differences in treatment effects were similar for all amines except for 5-HT in which s.c. injection produced a slight reduction, while i.p. injection resulted in a two-fold increase.(ABSTRACT TRUNCATED AT 250 WORDS)
用杀虫脒（CDF）、其两种N-去甲基代谢物（DMC和DDC）、对氯苯丙胺（PCA）和骆驼蓬碱处理大鼠后，采用配备荧光或电化学检测系统的高效液相色谱法分离全脑和血浆样本中的生物胺水平。皮下注射后1小时，CDF（200mg/kg）导致大鼠全脑样本中去甲肾上腺素（NE）、5-羟色胺（5-HT）和酪胺（TRM）水平降低，多巴胺（DA）水平升高，β-苯乙胺（PEA）水平无变化，而DMC（100mg/kg）和DDC（25mg/kg）使脑内NE、5-HT、TRM和PEA水平降低，DA无变化。定期跟踪DMC（100mg/kg）对脑内NE和DA水平的影响，持续24小时。在1小时显著下降后，NE水平在12小时升至最高，并在24小时测试期的剩余时间内一直高于对照组。DA水平在最初12小时内升高，并在剩余12小时内显著高于对照组。研究了皮下注射与腹腔注射DMC（100mg/kg）对脑胺水平的影响。腹腔注射处理通常导致DMC和赋形剂处理动物的胺水平较低。除5-HT外，所有胺类的处理效果差异相似，其中皮下注射使5-HT略有降低，而腹腔注射导致其增加两倍。（摘要截断于250字）
